UV excitable immunoglobulin labels

• Messages sorted by: [ date ][ thread ][ subject ][ author ]
• Next message: Dr. KH Leong: "IL-5R source"
• Previous message: Dave Coder: "ISAC XVIII Abstracts"

In current work with a UV-excitable pyrene derivative
(EX 351 / EM < 500 nm) we would like to combine analysis
with a long wavelength (>>> 500 nm) emitting dye which
can be coupled to antibodies.

AMCA works fine at 351 nm but its emission wavelength is
too low. Any suggestions regarding alternatives?

I remember that a company called ULTRA provided UV-excited
680-nm and 720-nm emitting antibody conjugates
(Ultralite 680 and Ultralite 720) a couple of years ago.
Has anyone experience with these dyes at 351 nm
or the current address of the company?

I also heard rumours that PerCP can be excited in the
low UV. Any experience?

Thanks for any comments,

Gregor Rothe

***********************************************************
Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany

Tel. +49 (941) 944-6208, or -6233
Fax +49 (941) 944-6202

Internet: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE
***********************************************************

• Next message: Dr. KH Leong: "IL-5R source"
• Previous message: Dave Coder: "ISAC XVIII Abstracts"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu