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{From: owner-cyto-sendout
{To: Cytometry Mailing List
{Subject: re: apoptosis by flow
{Date: Wednesday, January 03, 1996 2:37PM
{
{Regarding the use of the subdiploid PI method to assess apoptosis
{by flow cytometry:
{
{There are two cell preparatory methods: one analyzes whole cells and
{the other lyses the cells and analyzes the released nuclei.
{
{Gating is critical to determine an accurate percentage apoptotic.
{
{Each tube contains "live" cells, apoptotic cells, debris, and
{clumps.
{
{I use a whole cell method with a linear fluorescence scale and
{use both LS gates [mainly to exclude debris] and FL gates [mainly
{to exclude doublets and clumps].
{
{Finally, you need to verify that this assay works for your system
{by alternate methods [counting the apoptotic cells under the scope
{is easiest and perhaps best].
{is easiest and perhaps best].
{is easiest and perhaps best].
{is easiest and perhaps best].
{
{Good luck,
{Tom Mc Closkey
{North Shore University Hospital
{Long Island, NY
{
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