DNA and RNA quantification in bacteria

Jaume Comas (jaume@giga.sct.ub.es)
Tue, 9 Jan 1996 17:22:57 +0000

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Hello, I am interested in reliable quantification of total DNA and
RNA in bacteria. We have tried RNAse digestion and staining with
Hoechst33342 or IP. We have not obtained consistent results. Our
feeling is that RNAse digestion is incomplete or there is an
important staining variability or something interfering
quantification.
Any help will be warmly wellcome. Thanks in advance,

Jaume Comas
Citometria/ S.C.T.
Universitat de Barcelona
Tel 34/3/402.13.46
Fax 34/3/402.13.98
E-mail: jaume@giga.sct.ub.es

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu