Re: 4 colour analysis on epics XL
Margaret Cooley (m.cooley@arnie.cfi.unsw.EDU.AU)
Thu, 25 Jan 1996 08:32:03 +1100
We have been routinely running 4 colour analysis on HIV patients on our XL
with FITC, PE, ECD and PerCP, although not with the combinations you
suggest. It works very well, with careful compensation, although it can
depend somewhat on the "brightness" of your antibodies. Compensating PE
out of ECD and vice versa seem to be the worst. We are usually using CD3
PerCP, CD4 or CD8 ECD and using PE and FITC for other markers of interest.
If you want more details, the person who does this work is John Zaunders:
he doesn't have internet access, but I could pass on messages.
Good luck
Margaret
Margaret A. Cooley, PhD
Centre for Immunology
St Vincent's Hospital
Darlinghurst NSW 2010 AUSTRALIA
Ph: 61-2-3617700; Fax: 61-2-3612391.
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