CD 34 QC

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We would appreciate any input on protocols for use in quality control of CD34
antibody. Our only known source of QC material is the KG-1a cell line,
available from ATCC....suggestions?

Many thanks

CR Conley, MD
Mayo Clinic Scottsdale
13400 E. Shea Blvd.

We have successfully used KG-1 rather than KG-1a. KG-1 cells are
approximately 2 logs brighter than isotype control using anti-CD34 from BD.

Ron Hill, Ph.D.
Sugen, Inc.
Redwood City, CA
ronald@sugen.sf.ca.us
415-306-7797 tel
415-369-0679 fax

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu