(no subject)
kukuruga%kasle1.dnet.wayne.edu@rocdec.roc.wayne.edu
Fri, 26 Jan 1996 13:41:56 -0500
Reply to Dr. Farkas...the easiest answer is that these tissues have been
fixed with formalin prior to raraffin embedding. This develops crosslinks which limits accessibility to the DNA by propidium iodide -- therefore, less dye
means less fluorescence, so your peaks will naturally be lower. Several
attempts at eliminating this have been reported -- the best of which I was from
a recent Cytometry article where the authors (I apologize for not remembering
the names...if it's necessary, I'll look it up -- I think it was Overton?) used
heating to elinimate the effect.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
