GFP and GAL

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The question is whether there is a red fluorogenic substrate for
beta-galactosidase activity monitoring. Yes, there is, but there seems to
be a lot of trouble with the resorufin based material. If you have access
to UV excitation I would rather recommend a chloromethylcoumarin-based
substrate. This one is available from Molecular Probes, Inc. (Tel: (541)
465-8300 / FAX: (541) 344-6504) under catalog number D-2921. Disclaimer:
I have not tried it myself.

Good luck !

Martin Poot

martin@probes.mhs.compuserve.com

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu