Re[2]:Eosinophils
tom_frey@bdis.com
Sat, 27 Jan 96 11:09:17
I just wanted to support Howard Shapiro's conjecture: "On a modern instrument
with high fluorescence sensitivity (Elite, FACSort,FACSCalibur) ... it may be
easier to sort on autofluorescence." On a FACSort or FACSCalibur, at least
with unstained fixed cells, it is quite easy to tell the eos from other cells
types in whole blood on the basis of autofluorescence. In fact the
autofluoresence difference is maintained in FL2 and FL3 as well as FL1, so
gating the diagonal small brighter cluster in FL2 vs FL1 is easier (visually)
than using a histogram. Throwing in a high-side-scatter criterion couldn't
hurt.
Warning: I can't swear that this is true for UNFIXED cells, and I haven't
looked at stream-in-air.
Tom_Frey@bdis.com
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