autofluorescence -Reply
Davin Jutila (DJUTILA@wpsmtp.siumed.edu)
Mon, 29 Jan 1996 11:11:25 -0600
Tony,
We have a investigator who is doing research with transgenic mice. He
also had high background problems. We found that the fixative was
causing all the background. So we aways used fresh cells, unfixed
and did a titer of the monoclonal to obtain optimal staining
characteristics.
Hope this helps,
Davin
Davin Jutila
Research Imaging/Flow Cytometry Facility
Southern Illinois University School of Medicine
Springfield, IL 62794-1220
email: djutila@wpsmtp.siumed.edu
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