Re: BRDU labeling of MNC.
James Weaver 301-594-5879 FAX 301-594-3037 (WEAVER@cder.fda.gov)
Tue, 06 Feb 1996 11:00:09 -0400 (EDT)
In response to Richard Meisters question on labeling of MNC with BRDU, I
might suggest that the lack of labeling is due to the lack of
proliferative activity by unstimulated lymphocytes. Unless the animals
are treated with a major antigenic challenge or a mitogen, little uptake
of BRDU can be expected either in vivo or in vitro. Hope this helps with
your problem.
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James L. Weaver Email: WEAVER@CDER.FDA.GOV
Molecular Pharmacology Phone: 301-594-5879
Division of Research & Testing FAX: 301-594-3037
OTR, CDER, Food & Drug Admin.
Laurel, MD
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