Eosinophil fluorescence stain

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I want to differentiate human eosinophils with use of fluorescence or
absorption in a flow cytometer. It seems to be quite difficult to
find such a stain, a stain like eosin (for bloodsmears) needs alcohol
and I do not want to mix the blood with other things then PBS-like
buffers.
Does anyone has a good idea?

Thanks.

Robbert van Leeuwen
Applied Optics engineer
University of Twente
The Netherlands

Robbert@top.utwente.nl

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu