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FISH Methodology
Istituto di Genetica
Universita di Bari
Via Amendola 165/A
70126 B , ITALIA
Laboratory Profile
Director: Dr. Mariano Rocchi
1. Facilities available: Molecular genetics laboratory; Sterile room for somatic cell hybrids cultures; Microscopes and digital imaging set-up (CCD camera).
2. Instrumentation: Axioplan microscope equipped with a cooled CCD camera; PowerMac computer and software for FISH image processing; In strumentation for molecular biology. Equipped sterile room for somitic cell hybrids cultures (used for producing sub chromosomal painting probes).
3. Specializition areas: Production of alphold centromeric probes specific for the centromeric regions of human chromosomes; Production of subchromosomal painting probes from somatic cell hybrids for FISH analysis of any cytogenetic rearrangement; Hundre ds of such probes are available at present.
4. Training Opportunities: FISH; Preparation of subchromosomal painting libraries from somatic cell hybrids; Handling of human-hamster somatic cell hybrids.
5. Special courses offered: Generation of somatic cell hybrids, Cytogenetic analysis of constitutional or acquired chromosome rearrangements through FISH analysis, with particular reference to subchromosomal painting probes.
6. Graduate Program: None.
7. Local Accomodations: Hotel Plaza, Piazza L. di Savoia, 15, Bari (85,000 Lit per night).
8. Transportation: Local airport is Bari Palese, with bus service to Bari.
9. Cost of Internships: Free.
10. Communication:
Dr. Mariano Rocchi
Istituto di Genetica
Universita di Bari
Via Amendola 165/A
70126 Bari, ITALIA
Phone: (39) 80 544 3371 FAX: (39) 80 544 3386 E-mail: rocchi@mvx36.csata.it
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