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This book presents a wide variety of methods useful for flow cytometry based laboratories. Methods are presented in recipe format without extensive explanation. The book allows the reader to make a reasonable attempt at setting up new assays without spending an excessive time reviewing the original literature to determine how to set up the assay required. Sections are generally divided into immunophenotyping methods, DNA/RNA methods, methods for cellular function, general methods, reagents and general laboratory principles. The book is very useful for laboratories that wish to begin to run assays for which they have little or no previous experience. The detailed reagent concentrations, recipes and techniques can significantly reduce the time taken to get a new assay running successfully. Space for annotations is available in most sections so that the user can customize their own copy to some extent.
• (Note: summary written by one of the authors)

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu