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This book is an excellent attempt at addressing the issue of application of flow cytometry to the field of Biotechnology. The 17 chapters are aimed at the scientists who utilize cell culture in the lab (nearly everyone!), as well as those who use microorganisms in their research (more each year!).

Five chapters are dedicated to microbial cytometry and cover viruses, fungi and bacteria. Several chapters deal with mounting antibody production and several chapters on physiological methods such as pH.

In general, the book is very refreshing. While the range of topics is intentionally narrow, the material covered is of high quality and up-to-date. For instance there is a nice chapter on "Antibody Secretion Assays Using Gel Microdrops and Flow Cytometry" - something I have not seen in any other books. Several chapters deal with the rapidly growing area of microbial cytometry - an area we are sure to see much more on in the future.

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu