Purdue University Cytometry Laboratories

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Confocal Microscopy Instrumentation

NOTE: This area still under construction

Image analysis Programs & Software

Bio-Rad MRC 1024 UV/Vis System
The MRC 1024 installed in the lab consists of the following components:
Lasers:

UV/Vis: Coherent Innova Enterprise Model 622 - 60 mW output (with remote control) Water Cooled
Visible: ILT Ar-Kr Model 5470K-00C-2B

Microscope:

Nikon Diaphot 300 (Inverted)
Objectives:
- Plan APO 10 x 0.45
- Fluor Phase 10 x
- Flour 20 x 0.75
- Fluor 40 x 1.3 DIC OIL
- Plan APO 60 x 1.4 DIC OIL
- Plan APO 100 x 1.4 OIL
DIC Condenser and optical components
EPI - Fluor Attachment with 100 w HG lamp, Powersupply for HBO 100w
Stage:
- Automated X/Y Stage
Z-Axis:
- Computerized Z-Motor model #31002 plus controller
Vibration Table:
- TMC Model 78-455-12 Size: 8' x 4', drilled 3/16 plate every 1"
Physiology Components:
- Heated Stage: DTC-W-120-LC Digital Temperature Controller & Stage Brook Industries made to order (see Picture)
- Objective Heater: OH-100 Objective Heater Brook Industries made to order

MRC-1024 Options:

3rd Detector Channel
Transmission Detector 3 color
Ethernet Link
Triple Labelling Filter

Computer System:

Compaq Prosignia 500 with 80 Meg ram, 21" Monitor

Networking:

Novell 4.01

Archival Storage/Backup:

Philips CD Rom Writer
Corel Optical disk

Output/Presentation:

Printers
Codonics NP1600 Dye Sublimation Color Printer
H-P 4M Laser Printer
Tektronics Color Laser Printer

Digital Camera

Lasergraphics LFR Personal

Workstations:

2 x Silicon Graphics Workstation (Indigo 2) (see data analysis above)

Bio-Rad DVC 250 Viewscan
The DVC 250 installed in the lab consists of the following components:

Lasers:

Visible: ILT Ar-Kr Model 5470K-00C-2B

Microscopes:

Nikon Diaphot 200 (Inverted) with computerized Z-Motor model #31002 plus controller

Nikon Labophot (Upright) with computerized Z-Motor model #31002 plus controller Camera:

Monochrome: Photonic Sciences "Darkstar"

Color: Optronics 470T Computer:

Compaq Prosignia 500 with 80 Meg ram, 21" Monitor Image Analysis Software:

Metamorph/Metafluor, Universal Imaging Corp. Video Monitor:

Sony HR Trinitron Filter Wheel:

Lambda 10-2, Suter Instrument Company Video Laserdisc:

Panasonic TQ-3031F Laser video disc Physiology Components:

Heated Stage: DTC-W-120-LC Digital Temperature Controller & Stage Brook Industries made to order (see Picture)
Objective Heater: OH-100 Objective Heater Brook Industries made to order

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For information contact J.Paul Robinson, Ph.D., Director PUCL, 1515 Hansen B050, Purdue University, West Lafayette, IN 47907-1515, U.S.A. Phone: (317) 494-0757; Fax: (317) 494-0517. Send an email message now to J.Paul Robinson: robinson@vet.purdue.edu robinson@flowcyt.cyto.purdue.edu

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu