Cell Biochemistry Martinsried
Flow Cytometer
PASIII Flow Cytometer and Cell Sorter (Partec, Münster, Germany)
Instrument Setup
- 5 photomultiplier channels
- 1 electrical sizing channel
- quarz objective and excitation light condensor
- incorporated air cooled 40mW argon ion laser (488nm)
- incorporated high pressure mercury arc lamp (HBO100)
- quarz fiber coupled 40mW HeCD laser (325/445nm)
- quarz fiber coupled 5W/100mW argon ion laser (488,514,354nm + other lines)
- glass fiber coupled 10mW HeNe laser (633nm)
- quarz fiber coupled 20mW KrFl excimer laser (254nm)
Analysis Parameters
- cell volume by hydrodynamically focused electrical sizing
- forward (FSC) and sideward (SSC) light scatter
- three fluorescence channels
- time channel
Sorting Capacities
- closed fluid piezo sorting chamber for simultaneous FSC/SSC/three fluorescence
cell sorting
- complex, non rectangular multiparameter window sorting
- see practical performance on Video
Data Display and Recording
- up to six parameter FCS1.0 or FCS2.0 list mode
- various one and two parameter display modes with plotting on cytometer computer
- network data transfer into DOS computers for multiparameter list mode analysis
and self learning data classification
For problems or comments, please contact:
G.Valet, E-mail:
valet@vms.biochem.mpg.de,
Max-Planck-Institut für Biochemie,
Am Klopferspitz 18a, D-82152 Martinsried, Germany,
Tel: +49/89/8578-2518, -2525, Fax: +49/89/8578-2563
INTERNET address: http://www.biochem.mpg.de/valet/cellbio.html
Last Update: Aug.28, 1996
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
