Re: Calcium analysis
Ray Hicks (rh208@cus.cam.ac.uk)
Sun, 11 Feb 1996 14:38:45 +0000 (GMT)
Hi Eric,
You can use FCS Assistant to calculate a ratio for you and output a line
graph of mean ratio versus time, as long as you can get your data across
from the HP to a macintosh. I'll step up my efforts to get it to save
in LYSYS-compatible format if you really want to analyse in LYSYS (but
the facilities are a bit limited, which is why I incorporated the line
graph option). If you've got an IBM it would be worth browsing Eric
Martz's Catalogue of Software, where there are some good "total
packages".
Ray
On Fri, 9 Feb 1996, Eric Miller wrote:
>
> Hi "Flowers(!)"
> Anyone out there doing Calcium analysis on a Facscan? I've been
> having a go using Fluo-3 and Fura red, both separately and in
> combination, with some fairly encouraging results. I use Lysys II on a
> Hewlett- Packard uprated 310 computer, but the analysis side is fairly
> tedious, since I need a 6 minute time course, and the *** computer won't
> do ratioing for me. Has anyone any thoughts about the best way to analyse
> and present these results, given that the analysis has to be done within
> Lysys II? I have tried to get assistance from B-D in the UK without success.
> Please help. If this doesn't work I have been threatened with a
> fluorimeter!
>
>
> Eric P Miller
> Edinburgh Medical Oncology Unit
>
> "I am Pentium of Borg. Division is futile......you will be approximated."
> Star Trek - Generations. Two Captains, one density.
>
>
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
