G0 cell sorting
ZBIGNIEW DARZYNKIEWICZ (darzynk@nymc.edu)
Thu, 16 May 1996 08:42:11 -0500
Discrimination of G0 lymphocytes from the cells progressing through
the cycle can be accomplished based on the differences in rhodamine
123 uptake combined with light scatter (Proc. Natl Acad. Sci USA
78:2383, 1981). Rhodamine 123 is not much toxic when the cells are
allowed to subsequently grow in its absence, and it does not affect
their cloning efficiency (Cancer Res.,42: 79, 1982).
Zbigniew Darzynkiewicz
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
