Labeled Virus

Dennis_Young@CIS.ucsd.edu
Wed, 7 Feb 1996 10:30:00 -0800

Is there a better way to follow virus binding than R-18 "dequenching"
(Leary,JF, Notter,MFD, Kinetics of Virus Adsorption to Single Cells Using
Fluorescent Membrane Probes and Multiparameter Flow Cytometry, Cell Biophysics,
4, 63-76, 1982.)?
Our first attempt failed to see any rhodamine fluorescence. What would be a
good positive control?
FACSTAR+, 514 nm excitation, 560 dichroic and 600 SHORTPASS in FL-2.

Dennis
*****************************************************************************
* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
*****************************************************************************


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu