NOTE: Conditions of use of this material: You may use these presentations in ANY WAY YOU WANT - BUT you cannot sell them, or transfer the copyright to any publisher. If you wish to publish any image/photo/graphic from this catalog, you may do so, by referring this paragraph to the publisher. No royalty is required unless the photo/graphic/image is being used for promotion of commercial materials. Books, journals, and comics are all permissible uses for these photos.

On-Line Tutorials:

The following two tutorials on fluorescence compensation and antibody conjugation were provided by Mario Roederer of Stanford University.

email: Roederer@Darwin.Stanford.Edu

Compensation Tutorial:


This tutorial discusses proper fluorescence compensation. There are two main "threads" on this tutorial. The informal discussion aimed at the less technically oriented operators, and a more rigorous treatment that provides additional examples and some math as well. The most recent version of this tutorial exists on the web and can be accessed directly if you are connected to the internet by pressing this symbol http://cmgm.stanford.edu/~roederer/compensation.

If you are not on the internet press to run the tutorial now on the CD ROM!


Fluorescence Conjugation of Antibodies Tutorial:

This tutorial provides a series of protocols for the conjugation of various fluorescent dyes to monoclonal antibodies. The most recent version of this tutorial exists on the web and can be accessed directly if you are connected to the internet by pressing this symbol http://cmgm.stanford.edu/~roederer/abcon.

If you are not on the internet press to run the tutorial now on the CD ROM!


Online Internet Tutorial

This tutorial is part of the Internet tutorial developed by Dr. Guenter Valet for the Rimini ISAC Congress. Go to the tutorial now .

Microsoft Powerpoint Tutorial:

Courtesy of Carlton Stewart, RPCI Laboratory of Flow Cytometry, Buffalo, NY

email:stewart@sc3101.med.buffalo.edu.

The following is a tutorial on fluorescence compensation and multi-color flow cytometry. It includes 49 individual slides in powerpoint format which can be downloaded and used for teaching, lectures, and study material.

Instructions:

1. Click on the highlighted filenames to see a composite image of what the PPT file contains.
2. Click on the "PC" or "MAC" icon to copy the file.
3. If you receive an error message, "OK" it, and proceed to provide the destination directory. The files should copy.
4. If this fails, copy the files directly from the CD ROM directory (d:\8\filename.ppt) to your hard-disk and open the files with Powerpoint. These files will be readable by both PCs and MACS (the actual files are PC here).

Click here for tutorial on flow cytometry (49 slides) (file:stewart.ppt)

Click here to view One sample slide, or Group 1, Group 2,

Group 3, Group 4

Macintosh Tutorials: UPDATED for Vol. 2

The following MAC tutorials were provided by Geoffrey Osborne, John Curtain School for Medical Research, Canberra, Australia

email:osborne@anu.edu.au

Set 1: Compensation Tutorial # 1 (2:MAC:COMP1SEA.HQX)

Discusses the intricacies of properly setting the compensation for multiple-color analyses on flow cytometers.

Set 2: Compensation Tutorial # 2 (2:MAC:COMP2SEA.HQX)

Discusses the intricacies of properly setting the compensation for multiple-color analyses on flow cytometers.

Set 3: Statistics Tutorial (2:MAC:STATSSEA.HQX)

Discusses statistics used in flow cytometry.

Set 4: Shutdown Protocol Tutorial (2:MAC:SHUTPROT.HQX)

Discusses statistics used in flow cytometry.

Set 5: CellQuest Tutorial (2:MAC:CQ_SEA.HQX)

A demonstration of the CellQuest for the Macintosh software.

Significant Sponsors of this CD:

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu