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I teach immunology with laboratory to college juniors and seniors (ages 20-21).
For many years, I have included an extensive flow cytometry exercise in the
laboratory course. We analyse (using WinMDI) normal mouse splenocytes and
thymocytes for subpopulations using surface markers by two-color direct and
indirect fluorescence. Each student does a different combination of
antibodies and cells. Each student is then asked to relate their results to
the class database. The entire listmode file databases for 1994 and 1995
(without analysis or comment; I was on sabbatical in 1996) are on the web at
http://www.bio.umass.edu/immunology/5flowcyt.html
which is a page under the class home page at
http://www.bio.umass.edu/immunology/mic540hp.html
which is under our immunology information site at
http://www.bio.umass.edu/immunology/immunology.html
You may also be interested in our flow cytometry web site at
http://www.bio.umass.edu/mcbfacs/flowhome.html
Its main claim to fame is my unique comprehensive catalog of free flow
cytometry software
http://www.bio.umass.edu/mcbfacs/flowcat.html
which is visited by about 800 people per month. In addition to WinMDI, you
may wish to take a look at the free MFI program which I wrote as it can do
time kinetic analyses, e.g. of calcium flux data, for which sample data
files are provided on the 5flowcyt.html page above.
I also created a page for immunology class resources on the web at
http://www.bio.umass.edu/mcbfacs/flowcat.html
It is still very small because I haven't found many resources to link.
It gets about 75 visitors/week on average.
BTW, I have corresponded with Naoum Salame of the French Ministry of
Education because we are both interested in educational molecular
visualization (see my RasMol Home Page URL below).
I will be interested in other responses you receive to your inquiry as I
would be interested in incorporating data of the sort you requested in my
class. Please let me know if you would like additional information, e.g. my
class lab handouts or some WinMDI graphics from the class data.
At 03:10 PM 1/7/97 EST, you wrote:
>
>Hello,
>I am a teacher in Biology and work in a French high school. I would like to set
>up, in the framework of the Institute of Pedagogic Research, teaching tools in
>Immunology from the software Win MDI. To that aim, I am looking for detailled
>cytometric data which demonstrate the basic principles in Immunology:
>- origin of lymphocytes (nude mice and irradiated mice);
>- site of maturation of B and T lymphocytes;
>- immune response to a viral infection (CD4 and CD8 lymphocytes and
>macrophages);
>- immune response to a bacterial infection (CD4, CD8 and plasmocytes).
>Thank you in advance for your help.
>Alain Pothet
>101617.1371@compuserve.fr
>
>
>
/* - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor (Immunology), Dept Microbiology, Univ Massachusetts,
Amherst MA US 01003-5720 413-545-2325 FAX:545-1578. RasMol Home Page,
http://www.umass.edu/microbio/rasmol; other web projects listed at
http://www.umass.edu/microbio/rasmol/em-web.htm
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web