Education in Immunology - Flow Lab Class

l.mclean@auckland.ac.nz
Thu, 9 Jan 1997 8:16:48 +1300 NZDT

Hi Eric,

Sounds like you have put an excellent program together.

Last year we set up a new Immunology paper for 90 third year undergraduate science
students (ages 18- 20 plus a few mature students). It has a total of 84 hours teaching
including 12 hours of practical laboratory classes.

One of the practical classes included interpretation of human flow data (relatively
straightforward stuff, PBMC from one normal and one leukemic, two colour with markers
defining major subsets only). They also looked at some blood and marrow smears,
purified PBMC using ficoll, and did some T cell E-rosettes to fill out the three hours of the
class.

But for the flow interpretation we just gave them the 2D dot plots and stats, rather than
getting them to start with the raw listmode files (let alone doing the staining or running
cells though the flow themselves - we didn't want to be too ambitious for the first year of a
new course, and having a class of 90 students can put big demands on eqipment).

I'd be most grateful for any other info and / or advice you could offer on this type of
exercise as a prac class. eg how long did they need? How much did they get done in the
formal class setting versus analysis, background reading etc outside formal class hours?
How did your students find it in terms of degree of interest? difficulty? workload (ours tell
us they are badly overworked but I guess they all say that!) ?

I am looking forward to checking out your web pages and software.

Could you possibly send me a copy of your class handouts and graphics, as mentioned in
your reply to Dr. Pothet (snail mail to the address below, or EMail attachment).

Many thanks,

Lachy McLean

------------- ********* --------------
Lachy McLean
Senior Lecturer in Clinical Immunology
Department of Molecular Medicine, University of Auckland
Private Bag 92019, Auckland 1, New Zealand
Tel: 64-9-373-7599 ext 6170 (office) ext 6199 (lab) Fax 64-9-373-7492
EMail: l.mclean@auckland.ac.nz


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