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I have a lab here that wants to sort sperm for PCR, ie. they're not
interested in X-Y determination, just sorting single sperm into
96-well plates for amplification. I was about to suggest staining
with Hoechst -342 (we have UV) but does anyone have a feel for (a)
the overall feasibility of this idea, and (2) whether Hoechst or
any other DNA stain will interfere with PCR?
While I'm in Pine, does anyone have any tipes for sorting chromosomes
on a 753? How bout when n is greater than 35?
Looks like it's gonna be an interesting year....thanks for any leads you
can provide.
Steve
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Steve G. Hilliard This space available,
Cell Analysis Facility email me for rates!
University of Georgia
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web