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You have very correctly questioned the attemps at alignment of morphologic
descriptions with specific immunophenotypes. To add fuel to the fire,
morphological discrimination implies reproducible sample preparation with
either minimal or very reproducible artifacts. Starting with Papanicolaou,
exfoliative cytology has employed wet fixation. Hematology has employed air
drying of blood and bone marrow films. Especially in the case of bone
marrow, where there is some tradition of looking at wet fixed, sectioned
material, it is well known that air drying changes morphology.
I would suggest that morphology and morphometric analysis might be more
useful if wet fixation were employed. The old analogy is that even though
Quantum Mechanics (Monoclonal Antibodies) is well developed, Newtonian
Physics (morphology) is still very useful. Good morphology should at least
provide a common sense test of molecular based diagnosis. I believe that
morphometric analysis (including human observation) and molecular cytology
should augment each other.
Robert C. Leif, Ph.D., PMIAC,
Vice President & Research Director
Ada_Med, A Division of Newport Instruments
Tel. & Fax (619) 582-0437
Please send e-mail to my new address, rleif@rleif.com
Thank you.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web