Two antigens plus DNA staining

Howard Ratech (ratech@aecom.yu.edu)
Tue, 14 Jan 1997 10:39:09 -0500 (EST)

Hi Flowers:

We have a project in which we would like to study DNA ploidy plus protein
expression of two nuclear antigens. Our intrumentation is limited to a
FACSCAN. I assume that we will start by using FITC and propidium idodide.
But what, if any, is your recommendation for a third color? Is this
feasible with the limitation of our instrument?

Thanks.

Howard Ratech, MD
Albert Einstein College of Medicine/
Montefiore Medical Center
Bronx, New York

ratech@aecom.yu.edu


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