Sorting insect cells?
Geoffrey Osborne (Geoff.Osborne@anu.edu.au)
Wed, 15 Jan 1997 13:52:35 +1100 (EST)
Hello,
We have been asked to sort virus infected SF9 insect cells. In our
initial attempt, all the sorted cells had ruptured as they are particularly
fragile when infected. Sorting was performed on a FACStar Plus, 70uM nozzle,
11 to 12 psi sheath pressure, low sample differential, with cells being
sorted into tubes containing suitable media. A larger nozzle size will be
used in the next lot of experiments
I'm interesting in hearing from people who have experience sorting
insect cell lines or other particularly fragile cells, and the strategies
you've employed to do this successfully.
Thanks in advance,
Geoff
======================================================================
Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|...
Phone: 61 6 249 3694
FAX: 61 6 249 2595
-----Surfing the Web?: Try http://jcsmr.anu.edu.au/facshome.html------
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