Hoechst Red fluorescence?

Ayala Sharp (lssharp@weizmann.weizmann.ac.il)
Thu, 16 Jan 1997 13:59:16 +0000

A paper in J. Exp. Med. Vol. 183 p. 1797 by Goodell et. al.
describes the isolation of Murine Hematopoietic stem cells
using Hoechst 33342 staining. They excite at 350nm and
measure fluorescence at two wavelengths using a 450 BP
(450/20) and 675 EFLP filters with a 610 dichroic mirror.
They show a nice plot of Hoechst Red versus Hoechst Blue.
The literature I've seen shows no Hoechst fluorescence in
the red region. Any comments ? Explanations ? Suggestions ?
Thanks, Ayala.

-------------------------------------------------------------------
Dr. Ayala Sharp
Biological Services,
Weizmann Institute of Science,
Rehovot, ISRAEL.
FAX: 972-8-9344113
E-mail: lssharp@weizmann.weizmann.ac.il


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu