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What volume of bone marrow do you aspirate for flow. If you aspirate too
much you get blood, not bone marrow. However, you want as much as possible
from a single site. I had thought 1mL per site was maximum but was told
today that 3 mL gives a good yield. Please share you specifications and I
will summarize for those interested.
Maryalice
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web