FITC staining for YEAST
Jihan Marjan (jmarjan@gpu.srv.ualberta.ca)
Thu, 6 Feb 1997 10:27:15 -0700 (MST)
I got this address from Dorothy at Medical Sciences Building,
University of Alberta, Edmonton.
The project invoves using S.C. yeast with a mammalian construct
inserted. We would like to confirm the presence of the mammalian
construct within the yeast using a FACS scan and antibodies against
epitopes iwthin the mammalian construct. Is the procedure for this
protocol the same as with mammalian cells with the addition of a 45 second
sonication step at the end before running a FACS scan?
I appreciate any comments you may have with regards to this
question?
Yours sincerely,
Jihan Marjan Ph.D.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
