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CYTOMETRY COURSE and WORKSHOP
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CONFOCAL IMAGING, FLOW CYTOMETRY, AND IMAGE ANALYSIS
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Krakow, 10-13 June 1997
Sponsored by Bio-Rad Company and the Jagiellonian University
=================================================================
For detailed information about this Course, please check:
http://www.if.uj.edu.pl/~ubdobruc or
http://helios.mol.uj.edu.pl
On-line registration:
http://helios.mol.uj.edu.pl/kurs/app.html
==================================================================
* PLACE:
-------
Jagiellonian University, Institute of Molecular Biology,
Krakow, Poland
* LANGUAGE: English
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* GENERAL OUTLINE:
-----------------
The Course and Workshop will embrace basic and some selected
specialized techniques in:
- confocal imaging
- flow cytometry
- image analysis
There will be:
LECTURES - open to all, no fee
PRACTICALS/TUTORIALS (experiments and discussion)
* TUTORS and LECTURERS:
-----------------------
Prof. G.J.Brakenhoff, University of Amsterdam, The Netherlands
Prof. Z.Darzynkiewicz, New York Medical College, USA
Prof. P.Robinson, Purdue Cytometry Laboratories, Purdue, USA
Dr J.V.Watson, Addenbrooke's Hospital, Cambridge, UK
Dr N.S.White, Department of Plant Sciences, Oxford University, UK
* PARTICIPANTS:
---------------
The Course is aimed at graduate students and young researchers from
areas of biology, biotechnology, medicine, chemistry, physics.
Participants are welcome to attend the lectures only (free of
charge) or register for practicals (tuition fee).
* LECTURES:
-----------
The lectures will cover:
CONFOCAL:
Principles of Confocal Microscopy
Choice of chromophores, laser lines, filters, lenses
in confocal microscopy
Photon statistics, sampling rate, confocal pinhole
- optimization of data collection
3-D image collection, restoration and analysis
Use of high NA oil and water immersion lenses
Resolution and contrast in confocal imaging
FLOW:
Principles of Flow Cytometry
Basic parameters: fluorescence, light scatter
Basic immunophenotyping using multicolour analysis
Cell cycle analysis, viability and apoptosis by flow
cytometry
Measurement of oxidative metabolism and cell
function
Flow measurements of platelet activation
IMAGE ANALYSIS:
Basic 2D image analysis
Image manipulation - noise, contrast, edge
enhancement, deconvolution
* PRACTICALS
------------
Practicals will cover:
CONFOCAL
Image acquisition - basic principles
Optimizing fluorescent image acquisition: role of
a confocal pinhole, sampling rate, etc.
Reflected light imaging
Multicolour image acquisition - bleedthrough
correction
Acquisition of 3D data sets
* Additionally:
We will offer a special tutorial on
microinjection of cells in culture
FLOW
DNA cell cycle analysis
Cell function by flow cytometry
Phenotyping
Multiparameter Data Analysis
Platelet activation
IMAGE ANALYSIS
Basic 2D image analysis
Image manipulation - noise, contrast, edge
enhancement
3-D image restoration and analysis
Deconvolution of confocal and wide field
fluorescence images
Participants of practicals/tutorials will gain hands-on experience
using:
>> a Bio-Rad MRC1024 confocal microscope
>> three Silicon Graphics workstations
>> BioRad, Beckton-Dickinson and Coulter flow
cytometers
The Course participants will study live and fixed
animal and plant cells.
Suggest your own topic for a tutorial
=====================================
The prospective students are encouraged to suggest subjects of
practicals that they will find most useful (refer to the
Application Form).
Bring your own samples
=====================
The participants are welcome to bring their own samples to be
studied during practicals (no clinical material, please).
Please note:
Enrollment for practicals is limited to 20 students
* TUITION FEE and DEADLINES
---------------------------
Deadline for applications is March 15.
The tuition fee, payable to the Jagiellonian University, is:
$100,- for graduate students,
$150, - for faculty members
- if received before March 15, 1997
The tuition fees received after March 15 will be $150 and $200,
respectively.
The tuition fee includes the course materials, handouts, a ticket
for a banquet. Hotel, meals, etc. are not included.
Please transfer the tuition fee to the following account:
Bank Przemyslowo-Handlowy, Oddzial IV Krakow,
Account no.: 10601389-1023-27000-400101
Please do _not_ _forget_ to include a note "Confocal
Imaging Workshop"!
* FINANCIAL SUPPORT, STIPENDS
-----------------------------
Financial support will be available to participants who registered
for the Course. Please contact dr Dobrucki for details.
* FREE CONFOCAL (http://helios.mol.uj.edu.pl/kurs.html#free)
------------------------------------------------------------
All students who successfully completed practicals on confocal
microscopy will be given 15 hours of use of the BioRad MRC1024
confocal microscope at the Jagiellonian University - free of
charge. These students will be welcome to continue their research
projects immediately following the Course or return at a later
date.
* ACCOMMODATION
---------------
A number of reasonably priced ($25-50//night) rooms have been
booked for the Faculty and Students at the Guest Houses of the
Jagiellonian University. Advance booking is required. Please
contact dr Dobrucki for details.
Please note:
June is a high tourist season in Krakow and only the pricey hotels
may have vacancies! Moreover, the Course coincides with a visit of
Pope John Paul II in Krakow. Early booking for University Guest
Houses is strongly recommended.
* COURSE CERTIFICATES
---------------------
Those who attended all Course lectures and successfully completed
at least four selected practicals\tutorials will be entitled to
receive a Course Certificate.
* REGISTRATION
--------------
Register on-line via our WWW page:
http://helios.mol.uj.edu.pl/kurs/app.html
or use the application form attached at the end of this document
* SIGHTSEEING AND SOCIAL PROGRAMME (more on our WWW page)
---------------------------------------------------------
The following trips are planned:
Museum of the Jagiellonian University (Collegium Maius)
featuring a unique collection of scientific equipment,
including old microscopes, copies of astronomical
instruments used by Nicolaus Copernicus, and more.
The medieval Salt Mine at Wieliczka
The Tatra Mountains
Krakow Old Town and the Royal Castle of Wawel
We are also planning a trip to a Museum at Auschwitz-Birkenau
(Oswiecim-Brzezinka) concentration camps.
* FURTHER INFORMATION:
---------------------
dr Jerzy Dobrucki
dobrucki@mol.uj.edu.pl
http://www.if.uj.edu.pl/~ubdobruc
fax. 48 12 / 33 69 07
tel. 48 12 / 34-14-42, ex. 268
48 12 / 34-16-80, ex. 268
address:
Jagiellonian University
Institute of Molecular Biology
Al. Mickiewicza 3
31-120 Krakow
================================================================
APPLICATION FORM
CONFOCAL IMAGING, FLOW CYTOMETRY, AND IMAGE ANALYSIS
Krakow, 10-13 June 1997
Please type in using CAPITAL LETTERS
First Name:.................................
Family Name:.................................
Occupation (undergraduate, graduate, post-doc, faculty,
other):.................
Institution:
full name............................
postal address:........................
telephone...............................
fax.....................................
electronic mail.........................
Name and address of your supervisor, group leader or laboratory
director:
....................
....................
....................
....................
....................
Prime interest (please mark with X):
... confocal imaging and image analysis
... flow cytometry
I want to attend (please mark with X):
... lectures only (free of charge)
... practicals and lectures (I will transfer the tuition
fee)
I suggest the following topics for tutorials/practicals:
1...................
2...................
3...................
4...................
Please enclose your curriculum vitae, including a list of
publications
---------------------------------------
Please return the form and cv by mail, fax or e-mail to:
dr J.Dobrucki
Jagiellonian University
Institute of Molecular Biology
Department of Biophysics
Al. Mickiewicza 3
31-120 Krakow
Poland
dobrucki@mol.uj.edu.pl
fax. 48 12 / 33 69 07
=======================================================
J.Dobrucki
Laboratory of Confocal Microscopy and Image Analysis
Department of Biophysics
Jagiellonian University
Al. Mickiewicza 3
31-120 Krakow
Poland
tel. (48-12) 34-14-42, ex. 268
fax. (48-12) 33-69-07
Internet: dobrucki@mol.uj.edu.pl
WEB: http:/www.if.uj.edu.pl/~ubdobruc
=======================================================
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web