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thanks to all both near and far who replied to my request for help on two
issues.
using Dpbs or Hpbs as the sheath fluid seems to be helping the cells recovery.
we haven't yet tried the other methods suggested including edta to detach the
cells or coated flasks to promote adherance.
thanks again
frank isdell
flow cytometry lab
the rockefeller university
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web