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I was curious if people had been successfully analysed GFP levels within
transfected cells using flow?
I once had an investigator try something like this (though I do *not* know
if it was GFP, per se) and the experiment was a complete bust! There was
something about the excitation/emmision that was not near instantaneous and
there was enough delay that no fluoresence could be detected except when
using a flourescent microscope.
David
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web