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If you start with a clean machine and provide consistent regular cleaning
then you will probably not have a problem.
For those who wish to clean only when things get dirty of contaminated
then they are going to have alot of fun. Once sheath lines are
contaminated by protozoa or bacteria (or even better the disconnects for
tubing) the only recourse is to replace all tubing and connectors. All
the decontaminates you can use will not get into every little nook and
cranny. The cost for replacing all of this is less than the antibodies
you will use to stain your samples.
Start clean and keep it clean.(regularly)
Jim Houston
St. Jude Children's Research Hospital
Cell and Gene Therapy
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web