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1) Does azide interfere with the DNAse enzyme activity? (we use azide in
our common stock of 1% paraformaldehyde solution)
2) Does the source of DNAse make a difference? (we have been using
molecular biology grade RNAse-free DNAse I)
Any other suggestions or helpful comments on common problems would be
greatly appreciated. We have already spoken with Dr. Sprent's lab and
they have not had similar problems.
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J. Paul Zimmer, Ph.D.
Developmental and Clinical Immunology
University of Alabama at Birmingham
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web