Macrophage membrane marker for counting % Macs in a sample???
Sims and Margie Adams (springer@clemson.campus.mci.net)
Mon, 3 Mar 1997 10:24:32 -0500 (EST)
I am attempting to lable Macrophages for identification with the flow.
However, I have not been able to find any comercial markers. Are there any
suggestions.
We are looking at determining the % of Macs in a perotenial sample and
from a spleen prep. in the deer mouse for use in a colormetric phagocytosis
assay. I realize phagocytosis can be assessed on the flow, however, my
advisor has chosen the colormetric relase of netural red, following
phagocytosis. My main problem then, is that the # of Macs in each sample
much be determined so that differences in OD readings are due to treatment
effects and not the concentration of MAcs. Handcounts could give us %
Macs, however, we will have 36 mice/day to assess this in while setting up
a PFC assay, Mitogenic Proliferation assays, and running the phagocytosis
assay and don't have time to preform hand counts on 36 samples. Therefore,
the most cost effective and quickest method needs to be found.
In the near future I will also need to assess phagocytosis and Mac% from
fish Kidneys (Red Drum), Quail spleens, and Alligator spleens.
Does anyone have any suggestions for a macrophage marker that would
possibly work with deer mice, red drum, bobwhite quail, or alligators.
Obviously something Mac specific but not species specific.
Please email any suggestions to springer@clemson.campus.mci.net
Thanks very much,
Margie M. Peden-Adams
Margie Peden-Adams
Research Assistant
The Institute of Wildlife and Environmental Toxicology (TIWET)
PO BOX 709/ 1 TIWET Dr.
Pendleton, SC 29670
864.646.2313(office/lab)
864.646.6270(office/home)
864.646.2277 (fax)
springer@clemson.campus.mci.net
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