Paraformaldehyde and autofluorescence.
IMMUNO-SHERBROOKE (immunolo@courrier.usherb.ca)
Wed, 5 Mar 1997 13:25:46 -0500
Dear colleagues,
I perform immunophenotyping of human lymphocytes by flow cytometry.
I use 1% paraformaldehyde solution (PFA; Sigma) in PBS for cell fixation.
The autofluorescence of fixed cell exited at 488nm is increased 6-7 fold
comparing to nonfixed control.
1. Can someone suggest me an alternative PFA source which gives
minimal autofluorescence?
2. Can someone suggest me a post-fixation protocol which reduces
PFA-fixed cell autofluorescence?
Thank you.
Dr. L. Volkov
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