detection of platelet microparticles
DAHL CHASE DIAGNOSTIC SERVICES (dcdsflow@mint.net)
Fri, 7 Mar 1997 14:58:03 -0500
To all flow colleges,
I have been asked by one of our pathologists to look into the detection of
platelet microparticles using flow cytometry (with CD42b) as a diagnostic
test for heparin-induced thrombocytopenia. He gave me an article by David
Lee et al: British Journal of Haematology, 1996, 95, 724-731 which includes
a procedure. However, as we all know, there is no such thing as a
straight-forward Flow procedure. So, my questions are:
1. has anybody had experience with this procedure/test and is willing to
share it?
2. is this a relatively easy procedure which can be incorporated into a
clinical lab (we do mostly DNA, CD4's, leukemia/lymphomas, and retics)
without spending too much time on getting the procedure "fine-tuned"?
3. what are the pitfalls?
4. Is the flow procedure widely accepted?
5. how is one reimbursed?
Thank you very much in advance for your responses
Andrea Illingworth
Dahl-Chase Diagnostic Services/Flow Cytometry
333 State Street
Bangor, Maine 04401
(207)990-4855
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
