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I have a rather simple sounding question, particularly for the sorters
out there. I am looking to positively separate CD4, CD8, and monocytes
from Ficoll-hypaque separated blood samples. I intend to use avidin
columns and biotinylated antibodies. Obviously CD4 and CD8 will be use
for the isolation of these lymphocytes, but the best monocyted marker is
less clear. I have considered CD14, CD15, and CD33. My goal is to
obtain a pure monocyte sample, not necessarily the entire monocyte
population. The cells will be fixed immediately after they are
isolated and the subsequent manipulations are not affected by the
activation state of the cells. Does anyone have experience with these
surface markers or others that would be more appropriate for isolating a
relatively pure monocyte population?
Thanks in advance
Wallace Lauzon
Dept. of Microbiology and Immunology
University of Ottawa
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web