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I'm looking for advice on the glycophoran A (GPA) assay used to look at M
and N allelic expression by flow. This is a new application for our
facility. We're currently using the BR6 assay described by Langlois et al
(Cytometry 11:513, 1990). However, we're having problems getting
consistent light scatter profiles and with the results in general. I think
part of the problem may be with the fixation.
Any suggestions or comments??? Thanks.
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Julie A. R. Pribyl
LeBien Lab/ U of MN Cancer Center
Lab phone: 612-626-4839
Flow Cytometry Phone: 612-624-7151
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web