RE: flow assay for hereditary spherocyto

Leary, James (jleary@mspo3.med.utmb.edu)
Wed, 26 Mar 1997 08:26:00 -0600

I would not expect light scatter pulse width to work. However, a
combination of forward scatter, side scatter and pulse rise-time
time-of-flight should work. Pulse rise-time is sensitive to the shape,
not just the diameter, of cells. If you have access to this measurement
on your flow cytometer, use start and stop thresholds of 5% and 35%
respectively for greatest sensitivity.

-----Original Message-----
From: Yashpal Agrawal [SMTP:yagrawal@messi.uku.fi]
Sent: Tuesday, March 25, 1997 5:43 PM
To: cyto-inbox
Subject: flow assay for hereditary spherocytosis

I tried to measure pulse width on a FACStar machine for this purpose. The
method was not able to differentiate from normal RBC's.
Hope this is useful.
Yash Pal Agrawal
Kuopio, Finland

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