Platelet Activation via 3-color analysis
Michael F. Wolf (michael.wolf@medtronic.com)
Tue, 01 Apr 1997 13:58:13 +0100
Looking for tips/feedback on doing a 3-color analysis of platelet
activation to compare degranulation (BD: CD62-PE) and gpIIb/IIIa
activation(BD: PAC-1-FITC) in heparinized human whole blood also
labeled with CD61 (BD: CD61-PerCP)as the pan-platelet marker.
Ultimately the purpose is to examine/compare platelet (and WBC)
activation events upon exposure of blood to common and surface-modified
versions of medical plastics used in cardiopulmonary bypass
applications. Any suggestions on optimal buffers and whether or not to
use fixatives? I'm somewhat new in the area but my limited experience
tells me that care is required in choice of the latter to avoid cell
lysis. M. Wolf
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
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