To-Pro-3 Iodide
carolyn.surdyk@chmcc.org
Tue, 1 Apr 1997 12:02:39 -0500
I am currently using To-Pro-3 Iodide from Molecular Probes
as my DNA counterstain for confocal microscopy on cytospun,
paraformaldehyde fixed thymocytes.
Even at 0.05 uM (1:20000 dilution from 1mM stock)
I still get interference between the To-Pro and a FITC
labelled secondary antibody to a nuclear protein.
Any suggestions? I can not use DAPI or PI as my counterstain
either.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
