Rochelle Diamond
Caltech Flow Cytometry Facility
diamond@cco.caltech.edu
Does anyone have any experience using DAKO's anti-PCNA-fitc in rat liver
cells? I am experiencing difficulty with inappropriate G1 staining in
these cells. We are using triton x-100 to wash out free antibody. This
system seems to work in human cells but upon switching to rat liver cells
there is a problem.
Any help or suggestions would gratefully be accepted.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
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