Acetone is used to fix frozen sections for immunoperoxidase.
This is a more gentle fixation than alcohol. You might try
this. Both alcohol and acetone will greatly affect the scatter
from your cells so do not expect them to fall in the same areas
of your cytograms. Also observe the preparation under a
microscope before putting it on the flow to determine whether
the cells are intact, how much debris is present, and whether
they are still fluorescent.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web