A fish lymphocyte called Wanda

Harbor Branch Oceanographic Institution (harbolon@CLASS.ORG)
Tue, 24 Jan 1995 14:07:33 -0800 (PST)

Hello to all! A basic question to all you fish immunologists out there.
How does one go about separating fish lymphocytes on ficoll hypaque for
flow analyses. I tried using the 1.070 ficoll hypaque, but the RBC's and
WBC's just sit on top of the gradient after centrifugation (they dont
enter the gradient) SO the gradient must be too dense! Can one dilute
ficoll hypaque and get the "right" density to achieve separation?

Thanks for any replies!

--Ross-- (blub blub)

Ross E. Longley, Ph.D. * Harbor Branch Oceanographic Inst., Inc.
Immunology Group Leader * 5600 U.S. Highway #1, North
Div. Biomed. Marine Res. * Fort Pierce, FL 34946
Phone (407) 465-2400, 486
FAX (407) 465-1523
e-Mail harbolon@class.org

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