Be aware, that all programs do not perform the subtraction the same
way. Multi2D calculates the percentage of cells with increased
probe incorporation. Other software programs just do a channel
by channel subtraction, which can lead to spurious results, and even
negative values.
Hope this helps
======================================================================
Brian Sailer
d9at@sdsumus.sdstate.edu
South Dakota State University
Broookings, SD 57007
In Alaska, if you ain't the lead dog the view never changes!
=======================================================================
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web