Cheer up - maybe it was an idea (assay) too far ahead of it's time.
FURTHUR.
- From Dennis Young:
Paranoia of RNAse's will help ensure good results. DEPC-treated sheath fluid, as
well as vigilant sample-tubing cleaning are a must.
See Cytometry 11:869-874 (1990).
(They used fixed cells, 5XSSC instead of the standard PBS and washed
sample-tubing with 10% Hydrogen Peroxide.)
tom d.
QUESTION AUTHORITY..............
on every fundamental precept proposed as an explanation of any
aspect of reality; because reality is a perception and therefore
inherently subjective and gaussian in distribution. (possibly Poisson)
(I said that.)
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 ==============================================================================
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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