> As recommended by N.J. Goulding, I read with great interest Koopman's 1994
> paper in Blood about surface staining apoptotic cells with annexin-FITC.
>
> I was wondering if anyone has tried this in conjunction with
> fluorescent immunophenotyping as a way of identifying which classes
> of lymphocytes are undergoing apoptosis?
>
> I am thinking of using the annexin-FITC along with various PE and CyChrome
> labelled CD markers. Any comments or suggestions?
>
> >From where can I buy some annexin-FITC already prepared?
>
> Jeff Clapper
>
> Iowa State University
>
>
>
>
Annexin V FITC was developed by Chris Reutelingsperger
<C.Reutelingsperger@BIOCH.RULIMBURG.NL>, it is
being supplied commercially by BRAND Applications BV, and requests for
information and supply can be made by fax +31 4499 2165 (Netherlands)
Ray
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web