re: Propidium Iodide Spectra

Marcia=Woda%Facs%UMASS@BANYAN.UMMED.EDU
Thu, 18 May 95 14:15:56 EDT

PI bound to DNA also excites very well with the UV lines of the argon laser.
While I understand that the bulk of the emission is in the red, you do get
signal around 425 nm so it has always bothered me when people want to throw
in PI to assess viability when performing dual beam experiments.


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu