There are competitive anti-idiotiypic antibodies which have a higher
affinity for the antibody on the bead than do the cell receptors. These
antibodies will displace the cells from the beads. Dynal sells a product
called Detachabead which works this way. Mix these antibodies with the
bead-cell mixture for one hour at room temp., with light agitation and the
beads release nicely.
> I've been trying to isolate CD8+ activated T cells expressing DR
>MHCII molecules on their surface using immunomagnetic beads. Unfortunately,
>the beads don't readily separate from the cells afterwards in culture
>overnight, and I often can't get enough cells for FACS analysis. Does
>anyone know of a gentle way of coaxing lymphocytes off from immunomagnetic
>beads - one that won't also alter the cells drastically, as I assume
>trypsin would? I would greatly appreciate any comments; please send them
>directly to :
>mvp@nih.gov. Thaks for your time.
>
>Mark Pavlick
***********************************************
Roger A. Burger E-mail: Roger@cpd2.usu.edu
Research Immunologist
Center for Persons with Disabilities
Utah State University
Logan, UT 84322-6895
Voice: 801-797-2042
FAX: 801-797-2044
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web